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. 2017 Jul 5;25(10):2394–2403. doi: 10.1016/j.ymthe.2017.06.028

Figure 4.

Figure 4

HOTAIR Promotes Trophoblast Invasion by Activating the PI3K-AKT Signaling Pathway

(A) Heatmap showing proteins with differential expression after HOTAIR overexpression. (B) KEGG pathway maps showed classification of differentially signaling pathway after HOTAIR overexpression. (C) western blot analysis of p-AKT ser473 and AKT expression in HTR-8 cells transfected with siCtrl, siHOTAIR-1, siHOTAIR-2, and control vector, or HOTAIR-overexpressing vector after 48 hr. (D) HTR-8 cells were transfected with control vector or HOTAIR- overexpressing vector after 48 hr, and the HOTAIR-overexpressing vector group was then treated with LY294002 for 2 hr. p-AKT ser473 and AKT expression levels were determined by western blot analysis. (E) HTR-8 cells were transfected with control vector or HOTAIR- overexpressing vector for 48 hr, and the control vector or HOTAIR-overexpressing vector group was then treated with LY294002 for 2 hr. The invasive ability of the cells was assessed by crystal violet staining (original magnification ×200).