Table 2. Measured parameters for the GTPase activity and polymerization of FtsZ from Methanocaldococcus jannaschii.
| Temperature | GTPase activity | Polymerization | ||
|---|---|---|---|---|
| °C |
kcat s−1 |
CC-GTPase μM |
kdepol s−1 |
CC-Pol μM |
| 40 | 174 ± 30 a | 5.5 ± 2.9 | 3.5 ± 0.2 | 3.9 ± 0.1 |
| 45 | 258 ± 36 | 4.7 ± 2.4 | ND b | ND b |
| 50 | 348 ± 18 | 3.3 ± 0.9 | 20 ± 2 | 1.8 ± 0.5 |
| 55 | 378 ± 42 | 3.3 ± 1.4 | 42 ± 2 | ND b |
| 60 | ND b | ND b | 120 ± 2 | 1.6 ± 0.5 |
| 65 | 432 ± 18 | 2.2 ± 0.5 | ND b | ND b |
| 70 | 552 ± 36 | 1.0 ± 0.4 | 360 ± 23 | 1.6 ± 0.4 |
| 75 | ND b | ND b | 540 ± 23 | ND b |
| 80 | 660 ± 54 | 1.1 ± 0.6 | 810 ± 25 | 1.1 ± 0.3 |
a. The uncertainties are the standard errors calculated with 68% confidence.
b. ND, not determined.