Fig 3. Summary of interaction data of MEX3C variants and mutants with AP-2.

Black boxes indicate sequences that were identical among MEX3C-1, MEX3C-2, and MEX3C-3. Thin gray boxes indicate sequences shared by MEX3C-1 and MEX3C-2. Thick gray boxes indicate sequences unique to MEX3C-1. Unfilled boxes indicate motifs and domains studied. Boxes filled with vertical lines indicate ubiquitin chains with lysine changed to arginine. Dashed lines indicate deleted regions in deletion mutants. Dashed red boxes indicate regions essential for MEX3C/AP-2 interaction. Sequence numbering is based on human MEX3C-1. “+” indicates able to pull down AP-2 complex in immunoprecipitation experiments while “-” indicates the inability to do so. The tag (Flag or HA) for each variant and mutant is indicated. Detailed immunoprecipitation data are presented in Fig 2.