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. 2017 Oct 5;12(10):e0185992. doi: 10.1371/journal.pone.0185992

Fig 7. MEX3C and AP-2 are involved in exosomal miR-451a secretion.

Fig 7

A. siRNA-mediated MEX3C or AP-2 inhibition reduced miR-451a in exosome-enriched preparations. For AP-2 inhibition, siRNAs for AP2A1 and AP2M1 were transfected simultaneously. Shown are representative data from two independent transfections. B. DOX-induction inhibited MEX3C expression at the RNA level (N = 3). C. shRNA targeting the MEX3C coding region inhibited MEX3C expression from pFlag-MEX3C-1 after DOX-induction (N = 2). Co-transfected EGFP expression was used as the control for transfection efficiency and loading. D. DOX-induced MEX3C inhibition decreased exosomal miR-451a but not miR-320a expression (N = 3). For B and D, the mean ± s.e.m. are presented. *, ** and *** indicate p<0.05, 0.01 and 0.0001 when compared with control in Tukey’s multiple tests (B) and Bonferroni posttests (D).