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. 2017 Oct 5;12(10):e0185992. doi: 10.1371/journal.pone.0185992

Fig 8. Role of AP-2 and MEX3C in exosomal miR-451a secretion.

Fig 8

A. HGS was efficiently inhibited by siRNA si-HGS-2 but not si-HGS-1. Right: Expression as measured by densitometry (normalized with β-actin expression). Shown are representative results from two experiments. B. Inhibiting HGS expression failed to decrease exosomal miR-451a expression (N = 2). C. GW4869 significantly inhibited exosomal miR-451a expression (N = 3). The mean ± s.e.m. are shown. * indicates p<0.05 compared with DMSO control in Bonferroni posttests following ANOVA. D. Proposed model for AP-2/MEX3C interaction. At least two regions in MEX3C are necessary for its interaction with AP-2 complex. The red question mark indicates uncertainty whether the MEX3C/AP-2 interaction is direct or indirect; the black question mark indicates that the identities of the substrates ubiquitinated by MEX3C ring finger domain, and the proteins that directly bind microRNA, are unknown. E. Proposed role of MEX3C in ceramide-mediated miRNA exosomal sorting. MEX3C increases the association of microRNA to the endosome. ILV: intraluminal vesicles; MVB: multivesicular body; *: ceramide. It is unknown whether MEX3C itself is sorted into the ILVs, so MEX3C was not shown in ILVs.