REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-GFP, affinity purified	Abcam	cat#ab6556; RRID: AB_305564
Mouse monoclonal anti-actin AC-40	Sigma-Aldrich	cat#A4700; RRID: AB_476730
Mouse monoclonal anti-Fmi #74	Developmental Studies Hybridoma Bank [14]	RRID: AB_2619583
Rat anti-Pk, affinity purified	David Strutt [49]	N/A
Rabbit anti-Dsh, affinity purified	David Strutt [49]	N/A
Rabbit anti-Stbm	Tanya Wolff [50]	N/A
Chemicals, Peptides, and Recombinant Proteins
16% paraformaldehyde solution (methanol free)	Agar Scientific	cat#R1026
Triton X-100	VWR	cat#28817.295; CAS: 9002-93-1
Glycerol	VWR	cat#284546F; CAS: 56-81-5
DABCO	Fluka	cat#33480; CAS: 280-57-9
Normal Goat Serum	Jackson Labs	cat#005-000-121
Halocarbon 700 Oil	Halocarbon Products	CAS: 9002-83-9
Experimental Models: Organisms/Strains
fmiE59	Tadashi Uemura [14]	FlyBase: FBal0101421
stbm6	Tanya Wolff [51]	FlyBase: FBal0062423
dshV26	Norbert Perrimon [52]	FlyBase: FBal0003140
dsh1	Bloomington Drosophila Stock Center	FlyBase: FBal0003138; RRID: BDSC_5298
pkpk-sple13	David Gubb [53]	FlyBase: FBal0060943
dgo380	Suzanne Eaton [25]	FlyBase: FBal0141190
shits1	Bloomington Drosophila Stock Center	FlyBase: FBal0015610; RRID: BDSC_7068
fz-EGFP	David Strutt [5]	N/A
P[acman]-EGFP-dsh	David Strutt [5]	N/A
pActP>STOP>dsh-ECFP	David Strutt [35]	N/A
pActP>STOP>FLAG-stbm	This paper	N/A
pActP>STOP>FLAG-pk	This paper	N/A
pActP>STOP>FLAG-dgo	This paper	N/A
UAS-Pk attP{VK00031}	This paper	N/A
tub-CFP-Rab5	Suzanne Eaton [54]	N/A
sqh-Clc::mCherry	Thomas Lecuit [55]	N/A
pUAS-Rab5S43N	Bloomington Drosophila Stock Center	FlyBase: FBti0150344; RRID: BDSC_42703
hs-FLP	Bloomington Drosophila Stock Center	FlyBase: FBti0002044; RRID: BDSC_6
FLP22	Bloomington Drosophila Stock Center	FlyBase: FBti0000785; RRID: BDSC_8862
Ubx-FLP	Bloomington Drosophila Stock Center	FlyBase: FBti0150334; RRID: BDSC_42718
Actin-GAL4	Bloomington Drosophila Stock Center	FlyBase: FBti0012293; RRID: BDSC_4414
tubulin-Gal80ts	Bloomington Drosophila Stock Center	FlyBase: FBti0027796; RRID: BDSC_7019
Actin>y+>GAL4	Bloomington Drosophila Stock Center	FlyBase: FBti0012290; RRID: BDSC_3953
Ubi-mRFP-nls	Bloomington Drosophila Stock Center	FlyBase: FBti0129786; RRID: BDSC_30852
Software and Algorithms
NIS Elements AR version 4.60	Nikon	N/A
Image Lab version 4.1	BioRad Laboratories	N/A
ImageJ version 2.0.0	https://fiji.sc	N/A
MATLAB_R2014b	Mathworks	N/A
GraphPad Prism version 7.0c	GraphPad Software	N/A
G∗Power version 3.1	http://www.gpower.hhu.de	N/A
Packing Analyzer	Suzanne Eaton [33]	N/A
Polarity measurement scripts (MATLAB)	David Strutt [5]	N/A