Antibodies |
|
Rabbit anti-GFP, affinity purified |
Abcam |
cat#ab6556; RRID: AB_305564
|
Mouse monoclonal anti-actin AC-40 |
Sigma-Aldrich |
cat#A4700; RRID: AB_476730
|
Mouse monoclonal anti-Fmi #74 |
Developmental Studies Hybridoma Bank [14] |
RRID: AB_2619583
|
Rat anti-Pk, affinity purified |
David Strutt [49] |
N/A |
Rabbit anti-Dsh, affinity purified |
David Strutt [49] |
N/A |
Rabbit anti-Stbm |
Tanya Wolff [50] |
N/A |
|
Chemicals, Peptides, and Recombinant Proteins |
|
16% paraformaldehyde solution (methanol free) |
Agar Scientific |
cat#R1026 |
Triton X-100 |
VWR |
cat#28817.295; CAS: 9002-93-1 |
Glycerol |
VWR |
cat#284546F; CAS: 56-81-5 |
DABCO |
Fluka |
cat#33480; CAS: 280-57-9 |
Normal Goat Serum |
Jackson Labs |
cat#005-000-121 |
Halocarbon 700 Oil |
Halocarbon Products |
CAS: 9002-83-9 |
|
Experimental Models: Organisms/Strains |
|
fmiE59
|
Tadashi Uemura [14] |
FlyBase: FBal0101421 |
stbm6
|
Tanya Wolff [51] |
FlyBase: FBal0062423 |
dshV26
|
Norbert Perrimon [52] |
FlyBase: FBal0003140 |
dsh1
|
Bloomington Drosophila Stock Center |
FlyBase: FBal0003138; RRID: BDSC_5298 |
pkpk-sple13
|
David Gubb [53] |
FlyBase: FBal0060943 |
dgo380
|
Suzanne Eaton [25] |
FlyBase: FBal0141190 |
shits1
|
Bloomington Drosophila Stock Center |
FlyBase: FBal0015610; RRID: BDSC_7068 |
fz-EGFP |
David Strutt [5] |
N/A |
P[acman]-EGFP-dsh |
David Strutt [5] |
N/A |
pActP>STOP>dsh-ECFP
|
David Strutt [35] |
N/A |
pActP>STOP>FLAG-stbm
|
This paper |
N/A |
pActP>STOP>FLAG-pk
|
This paper |
N/A |
pActP>STOP>FLAG-dgo
|
This paper |
N/A |
UAS-Pk attP{VK00031} |
This paper |
N/A |
tub-CFP-Rab5 |
Suzanne Eaton [54] |
N/A |
sqh-Clc::mCherry |
Thomas Lecuit [55] |
N/A |
pUAS-Rab5S43N
|
Bloomington Drosophila Stock Center |
FlyBase: FBti0150344; RRID: BDSC_42703 |
hs-FLP |
Bloomington Drosophila Stock Center |
FlyBase: FBti0002044; RRID: BDSC_6 |
FLP22 |
Bloomington Drosophila Stock Center |
FlyBase: FBti0000785; RRID: BDSC_8862 |
Ubx-FLP |
Bloomington Drosophila Stock Center |
FlyBase: FBti0150334; RRID: BDSC_42718 |
Actin-GAL4 |
Bloomington Drosophila Stock Center |
FlyBase: FBti0012293; RRID: BDSC_4414 |
tubulin-Gal80ts
|
Bloomington Drosophila Stock Center |
FlyBase: FBti0027796; RRID: BDSC_7019 |
Actin>y+>GAL4
|
Bloomington Drosophila Stock Center |
FlyBase: FBti0012290; RRID: BDSC_3953 |
Ubi-mRFP-nls |
Bloomington Drosophila Stock Center |
FlyBase: FBti0129786; RRID: BDSC_30852 |
|
Software and Algorithms |
|
NIS Elements AR version 4.60 |
Nikon |
N/A |
Image Lab version 4.1 |
BioRad Laboratories |
N/A |
ImageJ version 2.0.0 |
https://fiji.sc |
N/A |
MATLAB_R2014b |
Mathworks |
N/A |
GraphPad Prism version 7.0c |
GraphPad Software |
N/A |
G∗Power version 3.1 |
http://www.gpower.hhu.de |
N/A |
Packing Analyzer |
Suzanne Eaton [33] |
N/A |
Polarity measurement scripts (MATLAB) |
David Strutt [5] |
N/A |