Fig. 1.

OD₃₅₀ studies of aggregation propensity (delta temperature values) of dmLT containing solutions after thermal stress in presence of different excipients. Average delta temperature value at which the OD₃₅₀ value reaches 0.1 absorbance unit for dmLT (0.15 mg/mL) in control buffer plus excipient vs. dmLT in control buffer alone (10 mM sodium phosphate, 150 mM NaCl, pH 6.0 with no additional excipient; highlighted box). The dmLT samples are shown in order of highest to lowest OD₃₅₀ (indicating highest to lowest stability in terms of aggregation behavior). Error bars represent the standard deviation from triplicate experiments. The inset shows the representative OD₃₅₀ thermal melt experiment of dmLT formulated in control buffer alone and control buffer in the presence of glycerol, mannitol and sorbitol. Excipients in green, yellow and red showed a large increase, moderate increase and low/no increase in stability, respectively. ^*For 15% glycerol, OD₃₅₀ value did not reached 0.1 absorbance unit and hence delta temperature could not be calculated.