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. 2017 Sep 19;6:e29156. doi: 10.7554/eLife.29156

Figure 3. Characteristics and validation of significantly DE genes in CD14+ cells (monocytes) of Fulani+P.fal relative to Fulani -P.fal.

(a) Classification of the most significantly DE genes in CD14+ cells (monocytes) of Fulani +P.fal relative to Fulani –P.fal (adjusted p-value<0.01, 171 genes). (b,c,d) qRT-PCR analysis of selected DE genes, with individual genes relative to –P.fal individuals (b), and combined genes relative to –P.fal (c) or Mossi (d) individuals (Genes: ALDOA, ARID3A, DUSP8, FES, FURIN, GAA, GAPDH, IRF3, IRF5, NFKBIE, OSM, RFXANK, SQSTM1, STRA13, TOLLIP, TRAF7), in validation cohort of Mossi –P.fal (n = 4), Fulani –P.fal (n = 10), Mossi +P.fal (n = 8) and Fulani + P.fal (n = 5) (mean ±SD) (Also see Figure 3—source data 1). (e,f,g) Cytokine levels in blood plasma of Mossi and Fulani individuals following P.falciparum infection. Uninfected (-P.fal) and Infected (+P.fal). n = 11 Mossi –P.falw, 11 Mossi +P.falx, 25 Fulani –P.faly, 9 Fulani + P.falz(e) Inflammasome cytokines IL-1β (mean ±SD; w vs x n.s. p>0.9999, y vs z ***p=0.0005, w vs y n.s. p=0.9285, x vs z **p=0.0081) and IL-18 (mean ±SD; w vs x n.s. p=0.4813, y vs z *p=0.0106, w vs y n.s. p>0.9999, x vs z n.s p=0.0947). (f) Pro-inflammatory cytokines IFNγ (mean ±SD; w vs x *p=0.0456, y vs z *p=0.0343, w vs y *p=0.0409, x vs z *p=0.0243) and IL-6 (mean ±SD; w vs x n.s. p=0.0862, y vs z n.s. p=0.3023, w vs y n.s. p=0.7166, x vs z *p=0.0359) (g) TGFβ (mean ±SD; w vs x n.s. p=0.5287, y vs z n.s. p=0.5735, w vs y *p=0.0122, x vs z n.s. p=0.2619, w and x vs y and z **p=0.0059) and IL-10 (mean ±SD; w vs x **p=0.032, y vs z n.s. p=0.0659, w vs y n.s. p=0.7154, x vs z n.s. p=0.2014)

Figure 3—source data 1. qRT-PCR analysis of selected DE genes in monocytes of validation cohort of Mossi –P.fal (n = 4), Fulani –P.fal (n = 10), Mossi +P.fal (n = 8) and Fulani +P.fal (n = 5) (mean ±SD).
elife-29156-fig3-data1.xlsx (41KB, xlsx)
DOI: 10.7554/eLife.29156.011

Figure 3.

Figure 3—figure supplement 1. Volcano plot of expression of individual genes in Fulani +P.fal (n = 2) relative to Mossi +P.fal (n = 3) CD14 +PBMCs (monocytes).

Figure 3—figure supplement 1.

Figure 3—figure supplement 2. ELISA of blood plasma in Mossi and Fulani individuals following P.falciparum infection.

Figure 3—figure supplement 2.

Uninfected (-P.fal) and Infected (+P.fal). (n = 11 Mossi –P.falw, 8 Mossi +P.falx, 23 Fulani –P.faly, 9 Fulani +P.falz.) a. IL-1Ra (mean ±SD; w vs x *p=0.0257, y vs z **p=0.0048, w vs y n.s. p=0.5673, x vs z n.s. p=0.4807). b. IL-1RII (mean ±SD; w vs x n.s. p=0.5305, y vs z *p=0.0149, w vs y n.s. p=0.6179, x vs z n.s. p=0.4807). c. IL-18BPa (mean ±SD; w vs x n.s. p=0.3100, y vs z *p=0.0149, w vs y n.s. p=0.2038, x vs z n.s. p=0.2766).