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. Author manuscript; available in PMC: 2018 Oct 5.
Published in final edited form as: Chembiochem. 2017 Aug 15;18(19):1928–1934. doi: 10.1002/cbic.201700343

Figure 1.

Figure 1

The expression and enzyme activities of TyrRS and its acetylated variants. A) SDS-PAGE and western blotting of purified TyrRS and its variants. Lane 1: wild-type TyrRS, lane 2: TyrRS-85AcK, lane 3: TyrRS-144AcK, lane 4: TyrRS-235AcK, lane 5: TyrRS-238AcK, lane 6: TyrRS-355AcK. The same volumes of elution fractions were loaded. B) ATP-PPi exchange assays of TyrRS and its variants. C) Aminoacylation assays of TyrRS and its variants. Mean values and standard deviations were calculated based on three replicates.