. Author manuscript; available in PMC: 2018 Oct 5.

Published in final edited form as: Chembiochem. 2017 Aug 15;18(19):1928–1934. doi: 10.1002/cbic.201700343

Table 1.

Kinetic parameters for Tyr activation by TyrRS and its variants.

	k_cat [s⁻¹]	K_M,ATP [mM]	K_M,Tyr [μM]	k_cat/K_M,ATP [s⁻¹mM⁻¹]	k_cat/K_M,Tyr [s⁻¹μM⁻¹]
wild-type	14.2 ± 0.7	0.43 ± 0.07	7.13 ± 0.81	33.02	1.99
85AcK	2.0 ± 0.3	2.23 ± 0.36	6.78 ± 0.65	0.90	0.29
144AcK	12.3 ± 1.1	0.45 ± 0.03	7.82 ± 0.27	27.33	1.57
235AcK	0.1 ± 0.0	0.71 ± 0.08	8.45 ± 1.02	0.14	0.01
238AcK	n.d.^[a]	n.d.	n.d	n.d.	n.d.
355AcK	11.9 ±1.7	0.49 ± 0.11	6.69 ± 0.43	24.29	1.78

^[a]

n.d.: not determined due to the undetectable activity of TyrRS-238AcK. Mean values and standard deviations were calculated based on three replicates.