. Author manuscript; available in PMC: 2018 Oct 5.

Published in final edited form as: Chembiochem. 2017 Aug 15;18(19):1928–1934. doi: 10.1002/cbic.201700343

Table 2.

Kinetic parameters for tRNA^Tyr aminoacylation by TyrRS and its variants.

	k_cat [s⁻¹]	K_M,tRNA [μM]	k_cat/K_M,tRNA [s⁻¹μM⁻¹]
wild-type	1.32 ± 0.23	0.37 ± 0.04	3.51
85AcK	0.15 ± 0.06	0.45 ± 0.05	0.33
144AcK	1.17 ± 0.37	0.42 ± 0.07	2.78
235AcK	n.d.^[a]	n.d.	n.d.
238AcK	n.d.	n.d.	n.d.
355AcK	1.09 ± 0.29	0.48 ± 0.14	2.27

^[a]

n.d.: not determined due to undetectable activities of TyrRS-235AcK and -238AcK. Mean values and standard deviations were calculated based on three replicates.