Table 1.

The Stern–Volmer quenching constants (K _sv), apparent static quenching constants (K _app), bimolecular quenching constants (K _q), binding constants (K _a) and relative thermodynamic parameters for the interactions of EGCG/theaflavins with BSA at different temperatures

Sample	Temperature (K)	F 0/F − [Q]	K (105 M−1)	K q (1013 M−1 s−1)	lg[(F 0 − F)/F] − lg[Q]a	K a (×106 L mol−1)	n	∆G 0 (kJ mol−1)	∆H 0 (kJ mol−1)	∆S 0 (J mol−1 k−1)
EGCGb	293	y = 22.64x + 0.6275 (R 2 = 0.9761)	2.77	2.77	y = 1.1242x + 6.6146 (R 2 = 0.9937)	4.12	1.12	−37.74	41.85	267.08
	310	y = 25.04x + 0.6478 (R 2 = 0.9817)	2.58	2.58	y = 1.1981x + 6.8986 (R 2 = 0.9913)	7.92	1.20	−35.44
TF1c	293	lny = 158771x − 0.0257 (R 2 = 0.9934)	1.59	1.59	y = 1.1523x + 6.2123 (R 2 = 0.9891)	5.16	1.15	−38.29	31.58	234.47
	310	lny = 166731x − 0.018 (R 2 = 0.9897)	1.67	1.67	y = 1.1312x + 6.3266 (R 2 = 0.9967)	8.45	1.13	−41.11
TF2Ac	293	lny = 309803x − 0.0128 (R 2 = 0.9825)	3.10	3.10	y = 1.2365x + 7.1449 (R 2 = 0.9979)	13.96	1.24	−40.76	15.43	188.55
	310	lny = 366957x − 0.021 (R 2 = 0.9899)	3.67	3.67	y = 1.2482x + 7.2496 (R 2 = 0.9975)	17.77	1.25	−43.02
TF2Bc	293	lny = 260913x + 0.0106 (R 2 = 0.9952)	2.61	2.61	y = 1.2455x + 7.0936 (R 2 = 0.9961)	12.41	1.25	−40.47	14.44	184.26
	310	lny = 284030x − 0.0144 (R 2 = 0.9841)	2.84	2.84	y = 1.2645x + 7.1916 (R 2 = 0.9899)	15.55	1.26	−42.68
TF3c	293	lny = 427620x−0.0343 (R 2 = 0.9867)	4.28	4.28	y = 1.2566x + 7.2297 (R 2 = 0.9897)	16.97	1.26	−41.24	15.89	191.72
	310	lny = 486809x − 0.1128 (R 2 = 0.9931)	4.87	4.87	y = 1.2733x + 7.3375 (R 2 = 0.9843)	21.75	1.27	−43.54

^aThe quencher concentrations ranged from 5 × 10⁻⁷ to 20 × 10⁻⁷ M

^bEstimated as K _sv from modified Stern–Volmer plot (Eq. 2)

^cEstimated as K _app from modified Stern–Volmer plot (Eq. 4)