Table 1.

Midpoint voltages and slope factors (k) of the activation and inactivation curves

Mutant	V 0.5,act; mV	K act, mV	V 0.5,inact; mV	K inact, mV
WT	1.8 ± 0.5 (n = 13)	6.6 ± 0.3	−18.3 ± 1.1 (n = 10)	14.1 ± 0.8
IS4 mutants
K1Q	13.2 ± 0.5 (8)	7.2 ± 0.5	−13.2 ± 3.5 (3)	17.4 ± 3.5
K1Q/R2Q	7.1 ± 0.8 (6)	9.4 ± 0.8	−20.3 ± 1.2 (3)	14.7 ± 1.4
K1Q/R2Q/R3Q	−11.9 ± 0.5 (10)	7.3 ± 0.3	−38.1 ± 0.8 (4)	8.2 ± 0.6
K1Q/R2Q/R3Q/R4Q	−20.9 ± 0.3 (10)	8.8 ± 0.3	−45.0 ± 0.7 (6)	6.2 ± 0.7
IIS4 mutants
R2Q	−7.8 ± 0.7 (3)	8.2 ± 0.7	−22.3 ± 3.6 (3)	13.9 ± 1.9
R6Q	−4.7 ± 0.4 (3)	7.5 ± 0.4	−29.7 ± 1.0 (3)	12.6 ± 0.7
R2Q/R3Q/R4Q/K5Q	−2.2 ± 0.5 (3)	7.0 ± 0.5	−27.8 ± 1.6 (3)	13.8 ± 1.0
R2Q/R3Q/R4Q/K5Q /R6Q	−3.3 ± 1.1 (3)	7.8 ± 1.0	−23.0 ± 1.3 (3)	15.4 ± 1.2
IIIS4 mutants
K1Q	0.7 ± 0.8 (3)	7.9 ± 0.7	−22.0 ± 3.0 (3)	12.0 ± 1.3
K1Q/R2Q/R3Q/R4Q	−1.8 ± 0.6 (4)	7.7 ± 0.4	−26.3 ± 1.9 (4)	11.3 ± 1.6
IVS4 mutants
R0Q	5.1 ± 0.3 (5)	7.7 ± 0.3	−17.7 ± 2.5 (5)	13.7 ± 2.3
R2Q	15.0 ± 0.4 (8)	5.9 ± 0.3	−0.9 ± 1.3 (3)	10.2 ± 1.2
R3Q	6.5 ± 0.3 (4)	6.1 ± 0.2	−21.3 ± 1.8 (3)	13.6 ± 1.6
R4Q	0.7 ± 0.6 (3)	5.7 ± 0.4	−22.9 ± 0.9 (3)	9.7 ± 0.7
K5Q	8.9 ± 0.3 (5)	6.5 ± 0.3	−5.6 ± 1.3 (3)	8.1 ± 0.7
R6Q	5.6 ± 0.4 (5)	8.3 ± 0.4	−20.8 ± 2.4 (3)	12.3 ± 1.9

Numbers of experiments are indicated in parentheses