Fig. 3.

Exoglycosidase sequencing of the FcγR N-glycans was used to identify the monosaccharide compositions and linkage information. Arrows indicate the migrations of peaks following exoglycosidase digestion and HILIC UPLC analysis A. Undigested FcγRI glycan profile B. Arthrobacter ureafaciens sialidase (ABS) C. bovine testes β-galactosidase (BTG) D. bovine kidney α-fucosidase (BKF), E. jack bean β-N-acetylhexosaminidase (JBH), F. jack bean α-mannosidase (JBM). Peaks migrate to Man₁ structures following digestion. Exoglycosidase sequencing was performed for each FcγR. The exoglycosidase panel digest for FcγRI is shown. See Supplementary Figs. for the Exoglycosidase panel digests for FcγRI, FcγRIIa_Arg131, FcγRIIa_{His 131}, FcγRIIIa_{Phe 158}, FcγRIIIa_Val158.