Table II. Summary of CHO expressed human FcγRs. Numbers are expressed as percentage values of the total glycan abundances.
FcγRI | FcγRIIa Arg131 | FcγRIIa His131 | FcγRIIb | FcγRIIIa Phe158 | FcγRIIIa Val158 | |
---|---|---|---|---|---|---|
Complex | 76.00 | 98.10 | 92.90 | 93.10 | 90.55 | 81.75 |
Hybrids | 7.10 | 1.30 | 2.10 | 3.80 | 4.95 | 9.30 |
High Mannose | 12.50 | 0.50 | 5.00 | 3.10 | 4.00 | 3.95 |
Total | 95.60 | 99.90 | 100.00 | 100.00 | 99.50 | 96.10 |
Antennarity | ||||||
mono (A1) | 3.40 | 0.80 | 4.00 | 2.20 | 4.20 | 2.90 |
bi (A2) | 32.00 | 54.50 | 50.55 | 41.90 | 31.90 | 43.45 |
tri (A3) | 27.20 | 25.05 | 23.70 | 30.35 | 25.75 | 25.50 |
tetra (A4) | 13.45 | 18.70 | 12.75 | 18.65 | 29.20 | 22.40 |
Galactosylation | ||||||
No Galactose (G0) | 25.15 | 4.80 | 10.5 | 8.30 | 7.15 | 11.40 |
mono (G1) | 23.80 | 5.60 | 15.70 | 19.60 | 10.05 | 18.30 |
di (G2) | 26.89 | 53.44 | 47.00 | 35.70 | 35.85 | 31.85 |
tri (G3) | 7.40 | 17.55 | 18.3 | 21.50 | 17.60 | 12.85 |
tetra (G4) | 12.40 | 14.50 | 9.50 | 14.90 | 28.85 | 21.70 |
Sialylation | ||||||
neutral (S0) | 55.60 | 67.90 | 85.65 | 81.70 | 54.40 | 57.65 |
mono (S1) | 26.60 | 25.80 | 8.10 | 10.1 | 23.30 | 24.90 |
di (S2) | 10.80 | 5.90 | 6.25 | 8.20 | 9.30 | 7.80 |
tri (S3) | 2.00 | 0.00 | 0.00 | 0.00 | 8.60 | 1.35 |
tetra (S4) | 0.60 | 0.30 | 0.00 | 0.00 | 3.90 | 1.40 |
Core-fucosylation | 74.50 | 95.00 | 89.35 | 76.15 | 73.90 | 78.75 |