Table I. High-confidence SOX2 interactors identified using BioID.

BioID was performed on two SOX2-BirA* biological replicates, each divided into two technical replicates for mass spectrometry. Spectral counts from each technical replicate are shown. Proteins scoring above a Bayesian False Discovery Rate of 1% (in this analysis corresponding to a SAINT score > 0.75) were considered high-confidence proximity interactors. Novel interactions (not reported in previous SOX2 AP-MS studies) are highlighted in blue.

^aControl runs consisted of five biological replicates of Flag-BirA*only that were each divided into two technical replicates. These 10 control runs were then collapsed to the three highest spectral counts for each hit.

^bSAINT = Significance Analysis of INTeractome confidence score. NA = not applicable.