Figure 4.

Successful mapping of TS lethal mutant strains via competitive fitness and liquid-format MIP-MAP techniques. Fifteen TS lethal mutants with genomic sequencing data were mapped by the competitive fitness approach used with hlh-1. Mapping results yielded a diverse group of mapping profiles including clean single-locus mappings as seen in VC50174 (A and B), profiles such as VC50383 with small additional loci or peaks appearing at later generations but resolved by additional liquid-format bulk segregant mapping of phenotypes (C and D), and multiple-locus profiles as observed in VC50182 (E) resolved to a single locus (F) by identifying shared background mutations at competing loci (G). Each line present in a graph represents mapping data from a different generation for the same experimental replicate or the results of a liquid-format bulk segregant mapping experiment (indicated as direct map with diamond points). MIP-MAP, molecular inversion probe mapping; TS temperature-sensitive.