Table 3. Table of temperature-sensitive mutants mapped.
| Strain | Set | WGS Coverage | Phenotype Penetrance | Timing of Lethality and Phenotype Observations at 26° |
|---|---|---|---|---|
| VC50022 | 3 | 16.6 | 100% | Arrest in early embryogenesis. |
| VC50028 | 3 | 40.7 | 100% | Sterility of P0 when plated at L4. Any F1s produced arrest at early larval stages. Some sterility also noted at permissive temperatures. |
| VC50031 | 3 | 16.4 | 100% | Sterility of P0 when plated at L4. Any F1s produced arrest at early embryogenesis. |
| VC50141 | 3 | 16.9 | 100% | Sterility of P0 when plated at L4. Any F1s produced arrest at early embryogenesis. |
| VC50174 | 1 | 21.9 | < 80% | Sterility of P0 when plated at L4, but also have bag phenotype so F1 are still produced. Failure of fertilization with vacuolated spermatozoa observed. |
| VC50178 | 1 | 20.7 | 100% | P0 when plated at L4 still produce progeny but F1 population is observed to be sterile. Severe morphogenesis defect, possible twofold arrest. |
| VC50182 | 1 | 19.8 | 100% | Early divisions normal, late arrest. Mostly twofold arrest with movement. |
| VC50255 | 1 | 20.0 | 100% | Sterility of P0 when plated at L4 with bag phenotype of dead eggs likely due to embryonic arrest. Any F1 produced are sterile. P1 delay is observed in embryos. |
| VC50260 | 1 | 22.9 | 99% | Sterility of P0 when plated at L4 with bag phenotype of dead eggs likely due to embryonic arrest. Any F1 produced are sterile. P1 delay is observed in embryos. |
| VC50352 | 2 | 23.4 | < 100% | Slightly leaky as P0 can give rise to F1, and then F2 but these are usually sickly and sterile. In some cases, they can make F3. Pretzel inviable, asynchronous cell cycle, later stage uncoordinated. |
| VC50360 | 2 | 22.2 | 100% | P0 have a mild roller phenotype, sluggish in appearance. F1 progeny appear to arrest in early embryogenesis. Pretzel inviable, pretzel deformed, cell adhesion/migration defect. |
| VC50374 | 2 | 20.9 | 99% | P0 when plated at L4 produce unhatched eggs of various forms and appears embryonic lethal. Any F1s produced are sterile. Arrest of development at premorphogenetic stage. |
| VC50375 | 1 | 19.0 | 100% | F1 sterility with P1 delay observed. |
| VC50380 | 2 | 19.6 | 100% | P0 when plated at L4 appear active and produce eggs. F1 progeny arrest in late embryogenesis and L1. Pretzel inviable, abnormal cytoplasm morphology, spindle defects, aberrant pharyngeal cluster and pretzel deformed. |
| VC50383 | 2 | 21.7 | 100% | P0 plated at L4 appear active but produce eggs that fail to hatch. Pretzel inviable, larval lethal, and muscle defects. |
WGS, whole-genome sequencing.