Table 5. TS Candidate mutation information.

Strain	Primary Candidate	Allele	Candidate Gene Phenotype Information	Examples of TS Allele(s) Reported Prior to This Sequenced Collection
VC50022	tbb-2	ax123	Early embryonic lethala	or362, or600
VC50028	mog-4	ax87	Sterilityb	q233
VC50031	emb-4	ax968	Larval lethalc	hc60
VC50141	ego-1	ax458	Embryonic arrest, and small oocytes with unusual chromosome morphology by RNAid.	None reported, in same exon as hypormorphic alleles om54 and om71
	zyg-9	ax3101	Embryonic lethal by RNAic,e,f. Failure to hatch in TS allele or623g.	or623, or628, or593, or635, or634
VC50174	C09D4.3	or976	No embryonic phenotypes reported. Peak expression in L4 and male samples. Expression present in sperm transcriptomeh	None reported
VC50178	let-805	or896	Embryonic lethale,f, maternal sterilec,e, larval lethalc,e,i.	None reported
VC50182	cts-1	or1005	Embryonic lethalc,e,i.	None reported
VC50255	pri-1	or1187	Delayed early cell division with prominent 3-cell stagej, and embryonic lethalitye,i.	None reported
VC50260	npp-8	or1044	Embryonic lethalc,e.	ax146
VC50352	pak-1	t3254	Low range embryonic lethal, larval lethal phenotypes and larval lethal with muscle detachmentk.	None reported
VC50360	sqt-3	t3352	Embryonic lethale, dumpye,f, roller phenotypesl by RNAi and various alleles.	e24, sc8, sc43, sc63, sc80
VC50374	taf-6.2	t3399	Embryonic lethalf	ax701, ax514
VC50375	par-5	or996	Embryonic lethalc,e, and cell cycle delayedm.	None reported
VC50380	let-2	t3320	Early larval lethal and embryonic lethaln.	mn114, e1470, b246, mn101, mn109, mn111, g30
VC50383		t3381

TS, temperature-sensitive; RNAi, RNA interference.

^aO’Rourke et al. (2011).

^bGraham et al. (1993).

^cKamath et al. (2003).

^dClaycomb et al. (2009).

^eSimmer et al. (2003).

^fSönnichsen et al. (2005).

^gBellanger et al. (2007).

^hMa et al. (2014).

ⁱRual et al. (2004).

^jEncalada et al. (2000).

^kZahreddine et al. (2010).

^lCox et al. (1980).

^mMorton et al. (2002).

ⁿMeneely and Herman (1979).