Figure 4.

Violinplot of Ka/Ks, Ka, Ks on SASTs in conserved AS events across syntenic genes within grass species. The pairwise Ka/Ks (A), Ka (B), and Ks (C) for SASTs flanking conserved AS events in five grass species were calculated and plotted. Only alignments >150 bp in length with gaps <5% of the total aligned length were considered. We only kept the Ka/Ks results if Ka < 0.5, Ks < 5 and Ka/Ks ratios < 2. The dotted lines within the violinplots represented the first, second, and third quartile. Wilcoxon Rank-Sum Tests were performed between different splicing event types (NS, Not significant; * P < 0.05, *** P < 0.001).