Skip to main content
. 2017 Jul 27;207(2):571–582. doi: 10.1534/genetics.117.300070

Figure 3.

Figure 3

CEP-1 promotes movement quiescence. (A) Time course of body-movement quiescence per 30 min period after irradiation at 1500 J/m2. [cep-1(gk138), N = 9; wild type, N = 10]. (B and C) Each point represents an individual animal’s total minutes of body-movement quiescence for 4 hr following irradiation divided by the mean quiescence of wild-type animals imaged simultaneously. P-values from t-tests comparing minutes of quiescence for each mutant group to the wild-type control group or between groups indicated by horizontal lines: * P < 0.05, ** P < 0.01, *** P < 0.001. (B) The N and mean for total minutes of quiescence for each group is as follows. cep-1(gk138): wild type (WT), N = 10, mean = 78.35; mutant, N = 9, mean = 46.48. cep-1(ep347): WT, N = 11, mean = 75.15; mutant, N = 11, mean = 86.85. cep-1(lg12501): WT, N = 10, mean = 101.5; mutant, N = 10, mean = 134.0. atl-1(tm853): WT, N = 11, mean = 92.91; mutant, N = 12, mean = 132.2. (C) Transgenic rescues of cep-1(gk138) using PCR-amplified wild-type DNA fragment (genomic) or a TransGenome Fosmid (transgenome). The N and mean for total minutes of quiescence for each group are as follows. Genomic: WT, N = 15, mean = 24.43; cep-1(gk138), N = 13, mean = 21.64; rescue, N = 15, mean = 24.74. Transgenome: WT, N = 14, mean = 86.04; cep-1(gk138), N=14, mean = 48.02; rescue, N = 14, mean = 75.05. (D) Schematic of CEP-1 indicating position of DNA binding domain, oligomerization domain (OD), and SAM domain. Below are representations of predicted mutant peptides created by each cep-1 allele with in-frame gap represented by dashed line for the gk138 allele. (E) Time course of body-movement quiescence per 30-min period in wild-type animals and cep-1(gk138) mutants upon overexpression of the EGF LIN-3C. (F) Total body-movement quiescence in the first hour after heat shock at 35° for 30 min. WT, N = 18; cep-1(gk138), N = 22. P-values from t-tests comparing minutes of quiescence: ** P < 0.01. In (B, C, and F), the wide and short horizontal bars denote means and SE, respectively.