Figure 2.

TB1 binds to pcna2 and tga1 promoters in vitro and in vivo. (A and B) Sequences upstream of pcna2 and tga1 where the probes bind the GG(G/C)CCC motifs. (C) EMSAs show that TB1 binds to the pcna2 and tga1 promoters via GG(G/C)CCC motif. Mutating the TB1 binding motif in the probe eliminates binding as seen by the lack of shifted bands. Each condition was replicated as paired lanes on the gel. (D and E) ChIP confirms that TB1 binds to the pcna2 and tga1 promoters in vivo. Input, total input chromatin DNA before precipitation; ChIP, chromatin DNA precipitated with anti-TB1 antibody PI, DNA precipitated with pre-immune serum; Mock, no antibody or serum added. (D) Agarose gel image of semi-qPCR amplification of the enriched promoter fragments. (E) qPCR with error bars indicating SEs.