Skip to main content
. 2017 Feb 10;31(10):2151–2160. doi: 10.1038/leu.2017.8

Figure 2.

Figure 2

CD5CAR NK-92 cells eliminate CD5 expressing T-ALL cell lines in vitro. (a) Co-cultures at an E:T ratio of 2:1 using CD5CAR NK-92 cells against malignant cell lines using CD56 and CD5 to delineate the NK cell and target cell populations respectively. Target cell survival in experiment is expressed relative to target cell survival in vector control NK treatment. Each bar graph represents the average statistics for duplicate samples with n=4. T-ALL cell line Jurkat was co-cultured for 6 h while all other cell types were cultured overnight. CD4 was used to identify negative control NHL cell line KARPAS cells. Populations encircled to show target cells of interest. (b) Co-cultures with CD5CAR NK-92 cells performed at an E:T ratio of 5:1 with the same experimental conditions. (c) Summary of CD5CAR NK-92 cytotoxicity against T-ALL and T-lymphoma cells lines. (d) Absolute cell counts of CCRF-CEM, MOLT-4 and Jurkat co-cultures of both effector and target cells. Control treatments are delineated in red while CD5CAR treatments are in blue.