Figure 1.

Accumulation of a CD11c⁺ cell population in the kidney of lupus‐prone mice. (a) Immunohistochemistry (IHC) stains of CD11c⁺ cells (red) on the kidney sections of 4‐month‐old Murphy Roths large (MRL) and MRL/lpr mice. Representative images are shown. Blue, 4',6‐diamidino‐2‐phenylindole (DAPI). (b) Stepwise gating of CD11c⁺ cells by flow cytometry as CD11c⁺CD45⁺Lin(CD3, CD19 and CD49b)^–CD11b⁺ cells from isolated kidney mononuclear cells from MRL/lpr mice. Representative flow cytometry plots are shown. (c) IHC stains of CD11c⁺ cells (red) and CD11b⁺ cells (green) on the kidney sections of 4‐month‐old MRL/lpr mice. Representative images of the medulla region are shown. (d–f) The percentages of renal‐infiltrating CD11c⁺ cells in Lin^– population (top row) as gated in (b) and the relative cell count changes of renal‐infiltrating CD11c⁺ cells (bottom row) in (d) 6‐week‐ and 15‐week‐old MRL/lpr mice, (e) 35‐week‐old New Zealand white (NZW) mice and NZB/W mice, and (f) 6‐week‐, 19‐week‐ and 37‐week‐old MRL mice. *P < 0·05; **P < 0·01; ***P < 0·001, Student's t‐test for (d,e) and one‐way analysis of variance (anova) for (f). Data are shown as mean ± standard error of the mean (s.e.m.); n = 3 mice in each group. [Colour figure can be viewed at wileyonlinelibrary.com]