Figure 2.

Phenotype and cytokine/chemokine profile of renal‐infiltrating CD11c⁺ cells. (a–f) The surface mean fluorescent intensity (MFI) of (a) major histocompatibility complex (MHC)‐II, (b) F4/80, (c) CD103, (d) CD115, (e) Lymphocyte antigen 6 complex (Ly6C) and (f) CCR2 on renal‐infiltrating CD11c⁺ cells (CD11c⁺CD11b⁺, red), CD11b^–conventional dendritic cells (cDCs) (defined as CD11c⁺CD11b^–MHC‐II⁺, blue), monocytes (defined as CD11c^–CD11b⁺Ly6C^highSSC‐H^low, orange) and neutrophils (defined as CD11c^–CD11b⁺Ly6C^midSSC‐H^high, green) from 4‐month‐old Murphy Roths large (MRL)/lpr mice as determined by flow cytometry. Representative flow cytometry histograms are shown. *P < 0·05; **P < 0·01; ***P < 0·001, one‐way analysis of variance (anova). Data are shown as mean ± standard error of the mean (s.e.m.), n = 3 mice in each group. (g) Relative transcript levels of selected cytokines and chemokines as determined by reverse transcription–quantitative polymerase chain reaction (RT–qPCR) in bone marrow monocytes [4',6‐diamidino‐2‐phenylindole (DAPI)^–CD11c^–CD11b⁺CD115⁺Ly6C^high], bone marrow neutrophils (DAPI^–Ly6G⁺CD11b⁺), splenic CD8⁺cDCs (DAPI^–CD11b^–CD11c⁺CD8⁺MHC‐II⁺) and kidney (KN)‐infiltrating CD11c⁺ cells (DAPI^–CD45⁺Lin^–CD11c⁺CD11b⁺) sorted from 4‐month‐old MRL/lpr mice. A heat‐map is shown. Red, higher expression level; green, lower expression level; n = 3 mice in each group. [Colour figure can be viewed at wileyonlinelibrary.com]