Table 1.
Primer | Sequence (5′–3′) | Product size (bp) |
---|---|---|
Human | ||
CD14‐F | TCCCTCAATCTGTCGTTCGC | 150 |
CD14‐R | ATTCCCGTCCAGTGTCAGGT | |
CD90‐F | GACCCGTGAGACAAAGAAGC | 138 |
CD90‐R | CCCTCGTCCTTGCTAGTGAA | |
TNF‐α‐F | CTTCTGCCTGCTGCACTTTG | 118 |
TNF‐α‐R | GTCACTCGGGGTTCGAGAAG | |
IL‐1β‐F | GTACCTGTCCTGCGTGTTGA | 153 |
IL‐1β‐R | GGGAACTGGGCAGACTCAAA | |
NGF‐F | CCCATCCCATCTTCCACAGG | 74 |
NGF‐R | GGTGGTCTTATCCCCAACCC | |
GAPDH‐F | TGTTGCCATCAATGACCCCTT | 202 |
GAPDH‐R | CTCCACGACGTACTCAGCG | |
Mouse | ||
TNF‐α‐F | CTGAACTTCGGGGTGATCGG | 122 |
TNF‐α‐R | GGCTTGTCACTCGAATTTTGAGA | |
IL‐1β‐F | GCAACTGTTCCTGAACTCAACT | 89 |
IL‐1β‐R | ATCTTTTGGGGTCCGTCAACT | |
NGF‐F | ATGGTGGAGTTTTGGCCTGT | 192 |
NGF‐R | GTACGCCGATCAAAAACGCA | |
GAPDH‐F | AACTTTGGCATTGTGGAAGG | 223 |
GAPDH‐R | ACACATTGGGGGTAGGAACA |
TNF = tumour necrosis factor; IL = interleukin; NGF = nerve growth factor; GAPDH = glyceraldehyde‐3‐phosphate dehydrogenase; bp = base pairs.