Figure 1.

Generation of type 1 regulatory (Tr1) cells. (a) Splenocytes from BALB/c wild‐type mice were phenotypically sorted into CD25⁻, CD25⁻ CD62L^hi CD44^lo (naive), and CD25⁻ CD62L^lo CD44^hi (memory) CD4⁺ T cells, and were cultured in the presence of interleukin‐10 (IL‐10) or IL‐27 cytokine. (b) In the presence of IL‐27, naive T cells were cultured with the addition of IL‐10, and CD25⁻ CD4⁺ T cells were cultured with the addition of α IL‐10 neutralizing antibody. Cells were stimulated with α CD3ε and α CD28 antibody for 3 days. Multicolour fluorescence staining was performed, and the stained cells were analysed by flow cytometry. Data are representative of three independent experiments; the values in the profiles are mean ± standard deviation (SD) from three independent experiments. Significant difference: *P < 0·05.