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. 2017 Aug 23;152(3):494–506. doi: 10.1111/imm.12786

Figure 2.

Figure 2

A lack of Ikaros leads to decreased expression of Foxo1 target genes. (a) Flow cytometry analyses measuring expression of CD62L and interleukin‐7 receptor α (IL‐7Rα) on the surface of CD4 splenic T cells from wild‐type (shaded histogram) and Ikaros null (black outlined histogram) mice. Bar graph is representative of mean fluorescence intensities compiled from three independent experiments. (*P = 0·0287, **P = 0·0055). (b) Quantitative RT‐PCR analyses of Sell and Il7ra expression using cDNA from wild‐type and Ikaros null (IK −/−) purified CD4 T cells. Values were normalized to levels of expression of the housekeeping gene, HPRT, for a minimum of six independent experiments. (*P = 2·84 × 10−5, **P = 3·61 × 10−5). (c) Quantitative RT‐PCR analyses of Foxo1, Sell and Il7ra expression using cDNA from wild‐type and Ikaros null purified splenic CD8 T cells. Values were normalized to levels of expression of the housekeeping gene, HPRT, for a minimum of five independent experiments (*P = 0·0016, **P = 0·0094, ***P = 0·00126). Error bars are representative of ± SEM.