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. 2017 Aug 21;3:17052. doi: 10.1038/cddiscovery.2017.52

Figure 2.

Figure 2

The effects of SAHA and TRAIL DR5 on CTSB expression. After starvation, 50% confluent MDA-MB-231 or MCF-7 cells plated in 6-well plate were transfected with 10 μM TRAIL DR5 siRNA targeting human TRAIL DR5 using lipofectamine 3000 reagent according to the manufacturer’s protocol. Then the cells were incubated with 5 μM SAHA for 24 h. Real-time PCR (a and d), Western blot (b, c, e and f) and ELISA (d and g) were used to access the expression of TRAIL-DR5 or CTSB. Relative quantitative values represent mean±S.E.M.; * represents statistical significance of P<0.05 comparing with Basal, ** represents statistical significance of P<0.05 comparing with SAHA.