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. 2017 Aug 21;3:17052. doi: 10.1038/cddiscovery.2017.52

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The effects of SAHA and TRAIL DR5 on cell viability and apoptosis of cancer cells. After transfected by TRAIL DR5 siRNA, MDA-MB-231 or MCF-7 cells were incubated with either 5 μM or 10 μM SAHA for 24 h, respectively. Fifty milliliters cell suspension from 2×10⁵ of collected cells was added with 450 μl count and viability reagent. Muse count and viability software module was used to analyze the results. For the apoptotic assay, 100 μl of Muse annexin V and dead cell reagent was added with 1×10⁶ cells for 20 min at room temperature. Muse cell analyzer was used to determine the percentages of alive, apoptosis, and dead cells. (a) The apoptosis profile in MDA-MB-231. (b) The cell viability profile in MDA-MB-231. (c) The cell count in MDA-MB-231. (d) The apoptosis profile in MCF-7. (e) The cell viability profile in MCF-7. (f) The cell count in MCF-7. Relative quantitative values represent mean±S.E.M.; * represents statistical significance of P<0.05 comparing with Basal.