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Disulfide analysis of Pfs230C1. Pfs230C1 containing four cysteine residues is expected to form two disulfide bonds. Using peptides from nonreduced trypsin and chymotrypsin digestion, >95% of the disulfide bonds were properly formed. Boxes indicate Cys 1-Cys 2 and Cys 3-Cys 4 disulfide bond formations with approximately 1 to 2% misformed Cys 2-Cys3 bonds and 3 to 4% misformed Cys 1-Cys 3 bonds.
