TABLE 1.
Characteristics of LC-MS analysis of disulfide-linked peptides from nonreduced Pfs230C1
| Protease | Retention time (min) | Monoisotopic m/z | MS area | Charge | Observed monoisotopic mass (Da) | Theoretical monoisotopic mass (Da) | Mass differencea | Disulfide-linked peptides |
|---|---|---|---|---|---|---|---|---|
| Trypsin | 28.8 | 963.2 | 3.3E+04 | 3 | 2,886.6 | 2,886.4 | 0.2 | V167-K173/E149-K166 |
| Trypsin | 29.7 | 887.4 | 1.0E+05 | 3 | 2,659.2 | 2,659.2 | 0 | K169-K173/E149-K166 |
| Trypsin | 30.8 | 1,266.6 | 9.4E+04 | 2 | 2,531.2 | 2,531.2 | 0 | C170-K173/E149-K166 |
| Trypsin | 30.8 | 844.8 | 1.7E+05 | 3 | 2,531.4 | 2,531.2 | 0.2 | C170-K173/E149-K166 |
| Trypsin | 26.4 | 581.3 | 8.4E+03 | 2 | 1,160.6 | 1,160.7 | −0.1 | C170-K173/I183-K188 |
| Trypsin | 46.8 | 1,046.1 | 2.1E+05 | 2 | 2,090.2 | 2,090.1 | 0.1 | I183-K188/A258-K269 |
| Trypsin | 39.8 | 914.2 | 3.3E+04 | 3 | 2,739.6 | 2,739.4 | 0.2 | I183-K188/E149-K166 |
| Trypsin | 46.8 | 697.8 | 2.0E+05 | 3 | 2,090.4 | 2,090.1 | 0.3 | I183-K188/A258-K269 |
| Chymotrypsin | 35.0 | 1,049.9 | 2.3E+04 | 3 | 3,146.7 | 3,146.6 | 0.1 | V151-F154/T155-L178 |
| Chymotrypsin | 35.2 | 1,049.9 | 1.8E+04 | 3 | 3,146.7 | 3,146.6 | 0.1 | V151-F154/T155-L178 |
| Chymotrypsin | 36.2 | 1,049.9 | 1.5E+05 | 3 | 3,146.7 | 3,146.6 | 0.1 | V151-L158/K159-L178 |
| Chymotrypsin | 35.2 | 1,049.9 | 7.3E+04 | 3 | 3,146.7 | 3,146.6 | 0.1 | V151-L158/K159-L178 |
a
The mass difference was calculated as the observed mass minus the theoretical mass.