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. Author manuscript; available in PMC: 2017 Oct 6.
Published in final edited form as: ACS Nano. 2017 Feb 14;11(3):2531–2544. doi: 10.1021/acsnano.6b08447

Figure 5. Preassembled translation initiation nanoplex enhances mRNA translation.

Figure 5

(A). A schematic of sucrose gradient fractionation to separate free mRNA, monosome, low molecular weight (LMW) and high molecular weight (HMW) polysomes. 24 hr after transfection of GFP mRNA or mRNA/eIF4E into HEK293T cells packaged with each of five polyamines, relative abundance of GFP mRNA in 12 fractions was measured by qPCR. Delivery of mRNA/eIF4E nanoplexes dramatically shifted the distribution of mRNA towards polysome-associated fractions relative to transfection with mRNA alone when packaged with (D) N3 (TET) and (F) N5 (PEH). In comparison, (E) N4 (TEP) enabled a marginal shift of actively translated mRNA when delivering mRNA/eIF4E and no such effects were detected with (B) N1 (EDA) and (C) N2 (DET). Results are representative data of two independent experiments.