Fig. 4.

A ketogenic diet upregulates antioxidative defense (SOD2), mitochondrial fission activity (FIS1), and a longevity-associated deacetylase (SIRT1) in mutUNG1-expressing mice. (A) SOD2 and the mitochondrial marker VDAC1 were quantified in Western blots, normalized to ActB as loading control. In mutUNG1 mice, SOD2 increases several fold compared with wild-type (p < 0.001) and in mutUNG1 mice it is doubled by a ketogenic diet (p = 0.03). VDAC1 shows only minor changes in the same conditions. (B) Similarly, FIS1 and SIRT1 are strongly upregulated by ketogenic diet in mutUNG1 mice versus mutUNG1 mice on standard diet (SIRT1 p = 0.006, FIS1 p = 0.007), as well as versus wild-type mice (SIRT1 p = 0.005, FIS1 p = 0.03); *p≤ 0.05; **p≤ 0.01. Abbreviations: SOD2, superoxide dismutase 2; VDAC1, voltage-dependent anion channel 1.