Fig. 8.

GABA_A receptor distribution in the CA1 region of the hippocampus. (A–C) Micrographs illustrating representative inhibitory synapses on the soma of CA1 pyramidal cells are shown for (A) wild-type, (B) mutUNG1-expressing mice fed a standard diet, and (C) mutUNG1-expressing mice fed a ketogenic diet Red arrows indicate gold particles (black dots) representing labeled GABA_Aγ₂ receptor subunits at the synaptic membranes (limited by black arrows). Scale bar = 100 nm (D and E) Quantification of inhibitory GABA_Aα₁ (D) and GABA_Aγ₂(E) receptor subunits. Gold particles within 25nmof the postsynaptic membranes were counted. The GABA_Aα₁ labeling showed a significant increase in mutUNG1-expressing mice fed a ketogenic diet (p = 0.02) compared with wild-type, whereas there were no significant differences in GABA_Aγ₂ labeling between the 3 groups of mice. Data are shown as mean subunit-representing particle number per µm²± standard deviation, n = 3 mice per group; *p ≤ 0.05. Abbreviations: KD, ketogenic diet; m, mitochondria; soma, cell soma; t, presynaptic terminal; v, synaptic vesicles. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)