Figure 9. Influence of separator refinement onto splitting adjacent glandular objects.

Ground truth is outlined in green, the segmentation result in blue. (A) segmentation without, and (B) segmentation with separator refinement. The separators emphasize the borders of the gland and provide a better input to the TV algorithm with more pronounced predictions, such that adjacent objects can successfully be split. If glands are very close and no significant visual cue for a border can be found, the separator refinement does not have any negative influence on the segmentation.