Table 1.
Steroid parameters for AB Sciex (Framingham, MA) API4000 QTRAP hybrid triple quadrupole/linear ion trap mass spectrometer tandem mass spectrometry monitoring quantitative (QN) and qualitative (QL) fragmentation patterns
| Steroid Common Name | Fragment Type |
Precursor Ion (m/z) |
Product Ion (m/z) |
Declustering Potential (V) |
Entrance Potential (V) |
Collision Energy (V) |
Cell Exit Potential (V) |
|---|---|---|---|---|---|---|---|
| Testosterone | QN | 289.1 | 97.2 | 100 | 15 | 30 | 12 |
| QL | 289.1 | 109.2 | 100 | 10 | 30 | 5 | |
| Testosterone-13C3 | QN | 292.6 | 112.2 | 75 | 10 | 40 | 5 |
| Androstenedione | QN | 287.2 | 97.2 | 100 | 15 | 30 | 12 |
| QL | 287.2 | 109.2 | 100 | 15 | 30 | 20 | |
| Androstenedione-13C3 | QN | 290.6 | 100.2 | 75 | 10 | 40 | 5 |
| Progesterone | QN | 315.1 | 97.2 | 75 | 15 | 25 | 10 |
| QL | 315.1 | 109.2 | 100 | 15 | 30 | 20 | |
| Progesterone-13C3 | QN | 318.4 | 112.2 | 50 | 10 | 35 | 5 |
| 17-OH-Progesterone | QN | 331.2 | 97.2 | 75 | 10 | 25 | 10 |
| QL | 331.2 | 109.2 | 100 | 15 | 35 | 20 | |
| 17-OH-Progesterone-13C3 | QN | 334.6 | 100.1 | 40 | 5 | 40 | 5 |
| Corticosterone | QN | 347.2 | 135.0 | 25 | 5 | 35 | 5 |
| QL | 347.2 | 121.3 | 40 | 5 | 20 | 15 | |
| 11-Deoxycortisol | QN | 347.3 | 109.2 | 25 | 5 | 35 | 10 |
| QL | 347.3 | 97.1 | 75 | 15 | 30 | 10 | |
| 11-Deoxycorticosterone | QN | 331.7 | 97.1 | 25 | 10 | 25 | 5 |
| QL | 331.7 | 109.3 | 75 | 10 | 35 | 5 | |
| Cortisone | QN | 361.4 | 163.3 | 100 | 5 | 35 | 12 |
| QL | 361.4 | 121.3 | 50 | 15 | 60 | 10 | |
| Cortisol | QN | 363.1 | 121.1 | 75 | 10 | 25 | 10 |
| QL | 363.1 | 267.3 | 25 | 5 | 30 | 30 | |
| Cortisol-d4 | QN | 367.4 | 121.3 | 75 | 10 | 25 | 10 |