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. 2017 Aug 1;31(15):1561–1572. doi: 10.1101/gad.301648.117

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© 2017 Wilhelm et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — Neuronal inhibition of bec-1 mediates health and life span effects. (A) Day 9 RNAi against members of the autophagic flux extended MTL in sid-1(pk3321);unc-119p::sid-1 worms. (B) Day 20 quantification of pharynx pumping rate in sid-1(pk3321);unc-119p::sid-1 worms. Data are depicted as a box plot with minimum to maximum values. RNAi against bec-1 was initiated at day 9. n = 50. (****) P < 0.0001. (C) Quantification of worm mobility measured by body bend counts in sid-1(pk3321);unc-119p::sid-1 worms at day 20. Data are depicted as a box plot with minimun to maximum values. RNAi against bec-1 was initiated at day 9. n = 50. (****) P < 0.0001. (D) Day 20 quantification of phalloidin-stained muscle damage following day 9 RNAi against bec-1 in sid-1(pk3321);unc-119p::sid-1 worms. The scoring system is the same as used in Figure 2D. n = 100. (****) P < 0.0001. (E) Day 20 quantification of longitudinal neuron morphology and integrity in unc-119p::GFP;rrf-3(pk1426) worms treated with RNAi against bec-1 at day 9. Neurons were scored on a three-point scale based on the amount of visible bubbling and neuron integrity. Neurons were quantified from images with 100× magnification. n = 100. Bar, 40 µm. (****) P < 0.0001. Days represent days after first egg lay. Life span statistics are in Supplemental Table 11.