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. 2017 Oct 6;12(10):e0185507. doi: 10.1371/journal.pone.0185507

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© 2017 Zhu et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — BXPC-3 and PANC-1 cells, respectively, were transfected with no plasmid/nanoparticle (Control group), 20 μg blank BSA-NPs to a final concentration of 2 mg/mL (BSA-NPs group), 20 μg of empty pcDNA3.1 using the lipid transfection reagent (Empty vector group), 20 μg of pcDNA3.1-hMDA-7 using the lipid transfection reagent (hMDA-7 plasmid group), and 20 μg of BSA- hMDA-7-NPs to a final concentration of 2 mg/mL (hMDA-7 BSA-NPs group), and assessed for apoptosis by flow-cytometry. A and C, flow-cytograms of BXPC-3 and PANC-1 cells, respectively; B and D, quantitation of A and C, respectively. *p<0.05 compared to Control, BSA-NP, and Empty vector groups; #p<0.05 compared to the hMDA-7 plasmid group. All experiments were repeated three times. Data are mean±SD.