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. Author manuscript; available in PMC: 2018 Feb 16.
Published in final edited form as: ACS Biomater Sci Eng. 2017 Feb 16;3(3):243–248. doi: 10.1021/acsbiomaterials.7b00055

Figure 4.

Figure 4

Representative images of tight junction protein ZO-1 after 4 days culture as the cells initially reached confluence on non-porous, 3.0 μm and 0.5 μm pore diameter SiO2 membranes, 2 MPa (Stiff) PDMS and 5 kPa (Soft) PDMS substrates. ZO-1 staining was most consistent at cell borders on 0.5 μm pore diameter SiO2 membranes and soft PDMS.