Figure 6.

Long-Range PCR and Southern Blot of Individuals with Multiple mtDNA Deletions and Control Individuals

(A) Long-range PCR of S3.

(B) Southern blot of proband S4. mtDNA was amplified with two primer pairs, giving 16,147 bp (F1) and 15,679 (F2) bp fragments. 5 μL of the amplified mtDNA was loaded onto a 0.7% agarose gel and separated at 80 V for 1 hr. Abbreviations are as follows: S3M, muscle of proband S3; S3F, fibroblasts of proband S3; S4, muscle of proband S4; M, marker λ/HindIII; C1–C3: control cells.