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. Author manuscript; available in PMC: 2017 Oct 6.
Published in final edited form as: ACS Biomater Sci Eng. 2016 Jul 25;3(8):1510–1518. doi: 10.1021/acsbiomaterials.6b00253

Figure 1.

Figure 1

Culture of hPSCs on microcarriers. (A) Human PSC seeding efficiency on microcarriers which underwent different treatment. Values are shown as mean±st.dev. from at least 3 independent experiments (* p<0.05 vs. all other treatments). VN: vitronectin; HSA: human serum albumin, UV: ultraviolet irradiation, PS: polystyrene (i.e. no treatment). (B) Human H9 hESCs are shown on VN− or VN+HSA-microcarriers on days 0 and 5 of culture. Cells were incubated with fluorescein diacetate for visualization. Bars: 500 μm. (C) Cell concentration vs. time for hPSCs culture on VN− (solid line) and VN+HSA-beads (dashed line). (D) Human PSC attachment on VN+UV and VN+HSA+UV microcarriers before (day 0) and after 4 days of culture in stirred suspension.