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. Author manuscript; available in PMC: 2017 Oct 6.
Published in final edited form as: ACS Biomater Sci Eng. 2016 Jul 25;3(8):1510–1518. doi: 10.1021/acsbiomaterials.6b00253

Figure 3.

Figure 3

Cells on VN+HSA+UV beads with neutral or positive surface charge cultured in stirred suspension are shown (A) at different culture times (FDA staining; bars: 200 μm) along (B) with their concentration (solid lines) and viability (dashed lines). (C) Concentration (solid line) and viability (dashed line) of hESCs cultured on VN+HSA+UV beads with neutral charge in stirred-suspension with E8 medium supplemented with pluronic F68. (D) Their expression of OCT4 and SSEA4 is also shown. Red curves: E8 supplemented with 0.02% F68, blue curves: E8 (no F68), Black curve: isotype control.