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Human ESCs (H9) expanded on VN+HSA+UV microcarriers in stirred suspension for multiple successive passages. (A) Cell growth (solid line), viability (dashed line) and (B) LDH activity profiles are shown. (C) Expression of pluripotency genes from the first (P1) to the last passage (P5) determined by qPCR. (D) Flow cytometric analysis of the fractions of OCT4+ and SSEA4+ hESCs. (E) Results of the karyotypic analysis of hESCs after 5 passages in microcarrier cultures.
