Table 1.
Primers used for qPCR assays in this study (shown in a 5′-to-3′ orientation).
| Gene | Forward primer | Reverse primer | Amplicon size (bp) |
|---|---|---|---|
| NANOG | AGATGCCTCACACGGAGACT | ACACAGCTGGGTGGAAAGAGA | 194 |
| FOXA2 | GAAGATGGAAGGGCACGA | CACGTACGACGACATGTTCA | 193 |
| SOX17 | CTTTCATGGTGTGGGCTAAGG | GTACTTGTAGTTGGGGTGGTCCT | 191 |
| PAX6 | AACAGACACAGCCCTCACAAAC | CGGGAACTTGAACTGGAACTGAC | 275 |
| ISL1 | GCGGAGTGTAATCAGTATTTGGA | CACTCGATGTGATACACCTTGG | 184 |
| KDR | CCAGAAGTAAAAGTAATCCCAGATG | CTTTAAAAGTTCTGCTTCCTCACTG | 246 |
| TUBB3 | TGTACTCCTTCCTGCTGGACTT | CCCCAACTCTCACTATGTGGAT | 270 |
| NES | CAGCGTTGGAACAGAGGTTG | GGGAATTGCAGCTCCAGCTT | 289 |
| SOX2 | TTTGATCCTGATTCCAGTTTGC | CAGCTCCGTCTCCATCATGT | 226 |
| POU5F1 | AAGCTGGAGAAGGAGAAGCTG | AATAGAACCCCCAGGGTGAG | 158 |
| GSC | AACCTCTTCCAGGAGACCAAGT | GACGACGTCTTGTTCCACTTCT | 182 |
| CXCR4 | ACTTTGGGAACTTCCTATGCAA | GCAAAGATGAAGTCGGGAATA | 217 |