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. 2017 Sep 26;13(9):e1007034. doi: 10.1371/journal.pgen.1007034

Fig 2. GLP/G9a complex-mediated H3K9 methylation counteracts Jmjd1a-mediated H3K9 demethylation in gonadal somatic cells.

Fig 2

(A) Flow cytometric analyses of E11.5 gonadal somatic cells for quantifying the H3K9 methylation levels. Cells were prepared from gonads with mesonephroi and then co-stained with antibodies against H3K9me2 and Nr5a1. Upper and lower boxes indicate the populations of Nr5a1-positive gonadal somatic cells and Nr5a1-negative mesonephric cells, respectively. (B) Representative histogram analyses for H3K9me2 levels of Nr5a1-positive gonadal somatic cells (upper) and Nr5a1-negative mesonephric cells (lower). (C) Statistical analysis of H3K9me2 levels of gonadal somatic cells of the indicated genotypes. Median fluorescence intensity (MFI) values for H3K9me2 of Nr5a1-positive gonadal somatic cells were normalized to those of Nr5a1-negative cells, and then plotted (n = 4–5). * P < 0.05; ** P < 0.01.