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. Author manuscript; available in PMC: 2017 Oct 6.
Published in final edited form as: Cell. 2017 Aug 10;170(4):787–799.e18. doi: 10.1016/j.cell.2017.07.044

Figure 5. R-Loops Disrupt Head-On Gene Expression, Related to Figure S4.

Figure 5

(A–C) qRT-PCR analysis from log phase cultures of either WT, ΔrnhC, ΔrnhC cells with rnhC expressed at an ectopic locus (ΔrnhC rnhC+) carrying the reporter gene lacZ (A) or luxABCDE (B). (C) Cells were induced and harvested in stationary phase. CD, co-directional (black bars); HO, head-on (gray bars). Data represent the means and SEM of at least three independent experiments. n.s., not significant. *p < 0.05.