Figure 4.

Lat Knockout Mice Present Morphological and Cellular Neuroanatomical Defects

(A and B) Schematic representation of brain regions modified in Lat^−/− animal models plotted in coronal planes according to p values at Bregma 0.98 mm (A) and −1.34 mm (B).

(C and D) Catalog of the 63 assessed brain measured in Bregma 0.98 mm (C) and −1.34 mm (D): 1, total brain area; 2, lateral ventricles; 3, cingulate cortex (Bregma 0.98 mm) and retrosplenial cortex (−1.34 mm); 4, corpus callosum; 5, caudate putamen (0.98 mm) and hippocampus (−1.34 mm); 6 = anterior commissure (0.98 mm) and amygdala (−1.34 mm); 7, piriform cortex; 8, motor cortex; 9, somatosensory cortex; 10, mammilothalamic tract; 11, internal capsule; 12, optic tract; 13, fimbria of the hippocampus; 14, habenula; 15, ventromedial hypothalamus; and 16, third ventricle. Green refers to length and black to area measurements.

(E and F) Histograms showing the percentage increase or decrease of measured brain regions in Lat^−/− mice as compared to the controls (100%) at Bregma 0.98 mm (E) and −1.34 mm (F). White coloring indicates a p value higher than 0.05 and gray to case subjects where the p value could not be computed due to missing data.

(G) Representative coronal brain images of wild-type (left) and Lat^−/− mice (right), stained with Luxol and Nissl, showing a significantly smaller area of habenula in knockouts when compared to matched wild-type.

(H) Areas in which cell counts were significantly affected. Bar graph shows total cell counts for WT (dark bars) and Lat^−/− animals (open bars). ^∗p < 0.05, ^∗∗p < 0.005.