Table 2. Peptides concentration as measured by using the Guinier’s approximation (first column); gyration radius RG (second column) and hydrodynamic radius (third column) as measured by SAXS and DLS; RG/RH ratio obtained by columns 2 and 3 (4th column); Calculated RG value for an ideal Gaussian Chain according to eq. 1 (5th coulmn).
| Peptide | Concentration mg/ml |
RG (nm) Guinier |
RH (nm) DLS |
RG /RH | RG (nm) RG =R0Nν |
RG (nm) EOM | A0.155/A0.97 |
|---|---|---|---|---|---|---|---|
| NH2htau | 2.5±0.1 | 1.32±0.03 | 0.95±0.05 | 1.4±0.1 | RG=1.18±0.06 | 1.31±0.05 | 3.1±0.2 |
| Reverse | 2.4±0.1 | 1.23±0.03 | 0.94±0.05 | 1.3±0.1 | RG=1.18±0.06 | 1.21±0.05 | 1.5±0.2 |
RG value computed by using the Ensable Optimization Method EOM (6th coulmn); A1.5/A1 values obtained by dividing the area of the peak at high RG by that of the peak at low RG value in Figure 8A.