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. 2017 Jul 18;8(39):64809–64826. doi: 10.18632/oncotarget.19363

Figure 1. Effect of combined FTI and SFN on HGPS cells.

Figure 1

A. Population doublings were calculated as stated in Materials and Methods for control and HGPS fibroblasts. Cells were either treated with vehicle (DMSO), SFN (0.25 μM or 1.0 μM), FTI (0.5 μM, 1.0 μM, or 1.5 μM), or combined drugs at the following concentrations: 0.25 μM FTI plus 0.25 μM SFN or 1.5 μM FTI plus 1.0 μM SFN for a period of 3 days. B. The same cells as in (A) were used to measure the levels of autophagy vacuoles by monodansylcadaverine (MDC) as described in Materials and Methods. Data are expressed as the mean ± S.D. (*p-value ≤ 0.05; n = 3). C. Immunohistochemistry of control (GMO1651C) and HGPS live cells (HGADFN127) using antibodies against monodansylcadaverine (MDC, autophagic vacuoles, blue) and propidium iodide (PI, viability, red). The percentages of dead cells (PI positive cells) are indicated and were determined by direct counts of an average of 900 cells for each treatment. Cells were treated as stated in (A). Representative images are shown (n = 3). Scale bar: 20 μM.